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Home >> Biotechnology and Genomics >> Biosensors as Biotechnology Tools >> Toxicology Tests using Biosensors

Toxicology tests using biosensors
A variety of pesticides and fungicides that are used for crop protection programmes, sometimes reach alarming levels in the atmosphere and become the cause of concern for human health care. These high levels of pesticides and fungicides are often detected through tests conducted on animals. Biosensors are being developed, which will spot dangerous levels of these chemicals in the atmosphere, thus obviating the need of animal testing.  Biochips carrying these biosensors are being tested by the British biotechnology company, Zeneca (manufacturer of agrochemicals). These biochips will indicate whether an experimental pesticide has reached a level, which is dangerous for humans, farm animals (including bees and earthworms) and plant life.

The biochips as above carry fragments of those genes from an organism, which are switched on due to exposure to high level of toxic chemicals (e.g., some genes that express in liver in humans). They are used to detect specific mRNA derived from these sensitive genes in the cell culture exposed to the chemical. The mRNA synthesized in the cultures exposed to chemical is labelled to facilitate detection. The technique has been successfully used by Zeneca to detect the presence of any fungicide using a biosensors with all 6000 genes from yeast. From humans also, 2000 genes, known to be activated by toxic drugs or pesticides, are being tested to select the best indicators.

 

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