Amperometric transducers
During amperometric measurement, a constant potential is maintained between a working electrode and a reference electrode. The current generated by oxidation or reduction of an electroactive species at the surface of the working electrode is measured. The signal generated is thus dependent on the mass transfer of the electroactive species to the electrode surface, so that a continuous use may cause fowling of electrode surface, causing loss of sensitivity. In view of this, even though amperometric biosensors are most successful, they could not make an impact on the market, where continuous use is required. Oxido- reductases are the best suited enzymes for these biosensors, since electron transfer is involved in transduction.