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Home >> Biotechnology and Genomics >> Bioinformatics and Data Mining - In Silico Biology >> Designing of PCR Primers

Designing of PCR primers

Computer programmes are also available for selecting PCR primer pairs that are each used to amplify a specific region of sequenced DNA. Primer (http://www.ganome.wi.mit.ebu) and Prima (http:\\www.emboss.org) are two such primer designing tools, although the latter is relatively less popular than PRIMER.

STS sequence search (program stssearch)
STS markers in some detail. These STS markers for different organisms are generalIy available in the databases (dbSTS). Genoniic and cDNA (EST) sequences of different organisms are also accumulating in databases (e.g. GenBank, dbEST, etc.) at a fast pace. Therefore, for the search of specific STS primer sequences in the genomic or EST databases, Program stssearch has been designed, which also helps in contig construction

 

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