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Home >> Biotechnology Dictionary >>Biotransformation - Buoyant density
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Biotransformation - the conversion of one chemical or material into another using a biological catalyst :a near synonym is biocatalysis, and hence the catalyst used is called a biocatalyst. Usually the catalyst is an enzyme, or a whole, dead micro-organism that contains an enzyme or several enzymes
Blunt end cut - to cleave phospho diester bonds in the backbone of duplex DNA between the corresponding nucleotide pairs on opposite strands. This cleavage process results in both strands finishing at the same residue, i.e., there are no nucleotide extensions on either strand. a.k.a. flush end cut
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Blastocyst - a mammalian embryo (fertilised ovum) in the early., stages of development, japproximately up to the time of implantation. It consists of a hollow ball of cells
Blunt end ligation - joining (ligation) of the nucleotides that are at the ends of two blunt ended DNA duplex molecules
Boring platform - sterile bottom half of a Petridish used for preparing explants with a cork borer
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Blastomere - anyone of the cells formed from the first few cleavages in animal embryology. The embryo usually divides into two, then four, then eight blastomeres, and so on
Blastula - in animals, an early embryo form that follows the morula stage; typically, a single layered sheet (blastoderm) or ball of cells (blastocyst)
Bound water - water held by the cell and not released if freezing occurs in the intercellular space. cf free water
Bovine spongiform encephalopathy - a.k.a. mad cow disease
Bract - a modified leaf that subtends flowers or inflorescences and may appear to be a petal
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Bleach - a fluid, powder or other whitening (bleaching) or cleaning agent, usually with free chlorine ions. Commercial bleach contains calcium hypochlorite or sodium hypochlorite, and is a common disinfectant used for cleaning working surfaces, tools and plant materials in plant tissue culture and grafting
Breeding value - in quantitative genetics, the part of the deviation of an individual phenotype from the population mean that is due to the additive effects of alleles. In practical terms :if an animal is mated with a random sample of animals from a population, that animal's breeding value for a certain trait is twice the average deviation of its offspring from the population mean for that trait
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Bleeding - used to describe the occasional purplish-black coloration of media due to phenolic products given off by (usually fresh) transfers
Blunt end - the end of a DNA duplex molecule in which neither strand extends beyond the other. a.k.a. flush end
Breeding - the process of sexual reproduction and production of offspring
Brewer's yeast - strains of yeast, often Saccharomyces cerevisiae, that are used in the production of beer
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Bovine somatotrophin (BST)
- a.k.a. bovine growth hormone, this protein is found naturally in cattle, and is the bovine counterpart of human growth hormone, one of the earliest biopharmaceutical products. It has been cloned, using recombinant DNA technology, expressed in large amounts and marketed as an agricultural product to improve the growth rate and protein:fat ratios in farm cattle, and to improve milk yield. Its use is banned in some countries
Breed - noun: in AnGR, either (i) a sub-specific group of domestic livestock with definable and identifiable external characteristics that enable it to be separated by visual appraisal from other similarly defined groups within the same species or (ii) a group of domestic livestock for which geographical and / or cultural separation from phenotypically similar groups has led to acceptance of its separate identity. cf breed at risk; breed not at risk; critical breed; critical maintained breed; endangered-maintained breed
Brewing
- the process by which beer is made. In the first stage the barley grain is soaked in water and allowed to germinate (malting), during which the natural enzymes of the grain convert the seed starch to maltose, and then to glucose. Grain is then dried, crushed, and added to water at a specific temperature (steeping) and any remaining starch is converted to sugar. The resulting liquid (wort) is the raw material to which yeast is added to convert sugar to alcohol. Hops (female flowers of Humulus lupulus) are added during this process to give a characteristic flavour
Bridge
- a filter paper or other substrate used as a wick and. support structure for a plant tissue in culture when a liquid medium is used
Broad host range plasmid
- a plasmid that can replicate in a number of different bacterial species
Broad sense heritability
- in quantitative genetics, the proportion of the total phenotypic variation due to genetic variation
Browning
- discoloration due to phenolic oxidation of freshly cut surfaces of explant tissue. In later stages of culture, such discoloration may indicate a nutritional or pathogenic problem, generally leading to necrosis
Bubble column fermenter
- a fermentation vessel, or bioreactor, in which the cells or micro organisms are kept suspended in a tall cylinder by rising air, which is introduced at the base of the vessel
Bud - a region of meristematic tissue with the potential for developing into leaves, shoots, flowers or combinations; generally protected by modified scale leaves. A terminal (or apical) bud exists at the tip of a stem or branch, while axillary (or lateral) buds develop in the axils of leaves
Bud scar - a scar left on a shoot when the bud or bud scales drop
Bud sport - a somatic mutation arising in a bud and producing a genetically different shoot. Bud sports includes changes due to gene mutation, somatic reduction, chromosome deletion or polyploidy
Budding - 1. a method of asexual reproduction in which a new individual is derived from an outgrowth (bud) that becomes detached from the body of the parent
2. among fungi, budding is characteristic of the yeast Saccharomyces cerevisiae
3. a form of grafting in which a single vegetative bud is taken from one plant and inserted into stem tissue of another plant so that the two will grow together. The inserted bud develops into a new shoot. See grafting
Buffer - a solution that resists change in pH when an acid or alkali is added, or when solutions are diluted
Buoyant density - the intrinsic density which a molecule, virus or sub cellular particle has when suspended in an aqueous solution of a salt, such as CsCl, or a sugar, such as sucrose. DNA from different species has a characteristic buoyant density, which reflects the proportion of G=C base pairs. The greater the proportion of G=C, the greater the buoyant density of the DNA
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