Dictionary of Autopolyploid, Dictionary of Axillary Bud Proliferation, Autoradiograph, Auxin-Cytokinin Ratio, Auxotroph

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Home >>Biotechnology Dictionary >>Autopolyploid - Axillary bud proliferation

Your Ad Here	Autopolyploid - a polyploid that has multiple and identical or nearly identical sets of chromosomes (genomes) all derived from the same species. A polyploid species with genomes derived from the same original species Autoradiograph - a picture prepared by labelling a substance such as DNA with a radioactive material such as tritiated thymidine and allowing the image produced by decay radiation to develop on a film over a period of time
Autoradiography - a technique that captures the image formed in a photographic emulsion as a result of the emission of either light or radioactivity from a labelled component that is placed next to unexposed film. The technique is used for detecting the location of an isotope in a tissue, cell or molecule. The sample is placed in contact with a photographic emulsion, usually an X ray film. The emission of b particles from the sample activates the silver halide grains in the emulsion and allows them to reduce to metallic silver when the film is developed. In genetic engineering, autoradiography is most commonly used to detect the hybridisation of a radioactive DNA (probe) molecule to denatured DNA in either the Southern transfer or colony hybridisation procedures
Autosome - a chromosome that is not involved in sex determination Auxin - a group of plant growth regulators (natural or synthetic) which stimulate cell division, enlargement, apical dominance, root initiation, andflowering. One naturally 'produced auxin is indole-acetic. acid (IAA) Axenic culture - free of external contaminants and internal symbionts; generally not possible with surface sterilisation alone, and incorrectly used to indicate aseptic culture, q.v Axillary bud - a bud found at the axil of a leaf (synonymous with lateral bud)	Your Ad Here
Auxin cytokinin ratio - the relative proportion of auxin to cytokinin present in plant tissue culture media. Varying the relative amounts of these two hormone groups in tissue culture formulae affects the proportional growth of shoots and roots in vitro. As the ratio is increased (in creased auxin or decreased cytokinin), roots are more likely to be produced, and as it is decreased root growth declines and shoot initiation and growth are promoted. This relationship was first recognised by C.O. Miller and F. Skoog in the 1950s
Your Ad Here	Auxotroph - a mutant cell or micro-organ-ism lacking the capacity to form an enzyme or metabolite present in the parental strain, and that consequently will not grow on a minimal medium, but requires the addition of some compound such as an amino acid or a vitamin for growth Axillary bud proliferation - propagation in culture using protocols and media which promote axillary (lateral shoot) growth. This is a technique for mass production (micropropagation) of plantlets in culture, achieved primarily through hormonal inhibition of apical dominance and stimulation of lateral branching

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Biotechnology Dictionary - Part 2 Cyclodextrin - Cytotype Dabs - Density gradient centrifugation Dexoyuribonuclease - Directional cloning Disaccharide - DNA micro-array DNA polymerase - Duplication Ecdysone - Encapsulation Encode - Euploid Evaluation - Exude F factor - Fusion gene Gelatin - Gram-positive organism Haploid cell - Homodimer Homogametic sex - Hypochlorite Immediate early gene - Insertion mutations Insertion site - Isozyme Kinetochores - Knockout

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