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Home >> Dictionary of Bioinformatics, Biochemistry, Biotechnology >> Microamplification - Microdroplet Array

MIAME
Minimum information about a microarray experiment. A convention for the unambiguous presentation of microarray data.

Micelle An aggregate of amphipathic molecules in water with the polar groups oriented outwards exposed to water and the nonpolar groups in the interior.

Michaelis constant (Km) The substrate concentration at which the velocity of an enzyme-catalysed reaction is half maximum.

Michaelis-Menten equation
An equation relating the rate of an enzyme-catalysed reaction to the substrate concentration. Michaelis-Menten kinetics A kinetic pattern in which the initial rate of an enzyme reaction shows a hyperbolic dependence on substrate concentration.

Michaelis-Menten kinetics
An Kinetic pattern in which the intial rate of an enzyme reaction shows a hyperbolic dependence on substrate concentratin

Michaelis Menten Kinetics

Michaelis-Menten equation

Michaelis Menten Kinetics

Micro (m) SI prefix, 10-6

Microaerophile
A microorganism that grows under conditions of very limited oxygen and is neither anaerobic nor aerobic.

Microalgal culture
Culture of microalgae in  bioreactors; microalgae include seaweeds.

Microamplification
A novel alternative in microcloning for the production of region specific chromosomal DNA. Single bands dissected from polytene chromosomes are digested with Sau3A followed by ligation of oligonucleotide adaptors to provide convenient priming sites for PCR amplification. This allows 1 microgram of DNA to be amplified from a single band. Microamplification is more effective than microcloning in providing probes for establishing chromosomal walks.

Microarray
A2D array, generally on a glass, filter, or silicon wafer, on which genes, gene fragments or oligonucleotides are deposited or synthesised in a predetermined spatial order allowing them to be made available as probes in a high-throughtput, parallel manner. It is used for comparative analysis and differential studies of genomes and transcriptomes. Also known as biochip or DNA chip.

MicroArray and Gene Expression Markup Language (MAGE-ML)

A language designed to describe and communicate information about microarray based experiments. MAGE-ML is based on XML and can describe microarray designs, microarry manufacturing information, microarray experiment setup and execution information, gene expression data and data analysis results. MAGE-ML has been automatically derived from Microarray Gene Expression Object Model (MAGE-OM), which is developed and described using the Unified Modelling Language (UML)-a standard language for describing object models.

Microarray technology
A method of analysing the interaction of large number of genes with each other and the simultaneous regulation of batteries of genes. A robot is used to apply tiny droplets of functional DNA onto glass slides. Fluorescently labelled DNA probes are allowed to hybridise to the complementary strands on the slides. A scanning microscope measures the brightness of each fluorescent dot on the slide. The intensity of fluorescence indicates the amount of a specific DNA fragment and thereby information whether it is overexpressed or downregulated.

Microautophagy
A lysosomal ubiquitin-independent protein degradation pathway in which proteins are internalized by the budding off of vesicles.

Microbe
A microorganism.

Microbial genetics
The study of genes and gene functions in microorganism.

Microbial mats
Layered groups of microbial populations.

Microbial mining
The use of microorganisms to remove minerals from rocks. There are two main areas in microbial mining: leaching, which involves use of bacteria to solubilise metals in ores, and purification, which involves use of microorganism to separate and concentrate metals from dilute solutions.

Microbodies
Cytoplasmic membrane-bounded vesicles that contain peroxide-forming and peroxide-catabolising enzymes such as lysosomes, glyoxysomes and peroxisomes.

Microcapsule
A very thin version of the capsule surrounding a bacterial cell wall. It is a gel like matrix made of polysaccharides that protects the bacterial cell from phagocytosis.

Microcarriers
Small particles used as a support material for cells, especially mammalian cells, which are too fragile to be pumped and stirred as bacterial cells are in a large-scale culture.

Microcells
Cell-like particles containing a single chromosome within a small nucleus surrounded by minimal cytoplasm and a cell membrane. These are generated by prolonged inhibition of mitosis followed by centrifugation.

Micrococcal nuclease
An endonuclease produced by Staphylococcus aureus that cleaves the covalent backbone of DNA strands. It is used for mapping protein binding sites of DNA.

Microcurie (mCi)
Ci × 10-6

Microdroplet array
A technique developed by Kao and Konstable to evaluate large numbers of media modifications, using small quantities of medium into which are placed small numbers of cells. Droplets of liquid arranged on the lid of a Petri dish, are inverted over the bottom half of the dish that contains a solution with a lower osmotic pressure and the dish is sealed. The cells or protoplasts form a monolayer at the droplet meniscus  and can be readily examined. Also termed multiple drop array (MDA); hanging droplet technique.

Microelectrode, micropipette
A piece of fine glass tubing that is drawn into a fine tip. It is used to penetrate a cell in order to study its physiology or to inject molecules or electric current.

Microelement
An element required in minute amounts.

Microencapsulation
The process of enclosing a substance in extremely small sealed capsules from which material is released by heat, or other means.

Microenvironment
The environment that is sufficiently close to the surface of a living or nonliving object so that it is influenced by it.

Microfibrils
A bundle of fine cellulose fibers that form the plant cell wall. These are extremely small fibres visible only at the high magnification of the electron microscope.

Microfilaments
Thin filaments made of actin present in the cytoplasm of eukaryotic cells. They play a role in structure and movement.

Microfluidics
The miniaturization of chemical reaction or pharmacological assays into microscopic tubes or vessels to enhance their throughput, by placing them side by-side in an array.

Microglobulin
A short peptide noncovalently bound to class l major histocompatibility complex glycoprotein.

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