Logo
 Home | Sitemap | Contact us | Search | Language
Left Right
Home >> Dictionary of Bioinformatics, Biochemistry, Biotechnology >>Limit Digest Lineweaver Burk Equation

Life cycle The complete sequence of events undergone by organisms of a particular species, staring from the fusion of gametes in one generation to the same stage in the next generation

LIGAND a database that stores data about chemical compounds and reactions occurring in the cell

Ligand A small molecule that binds noncovalently to another molecule (an enzyme or receptor) in or on cells

Ligand-gated ion Channel An ion channel that opens in response to the bindings of a specific ligand (eg. glutamate and γ-aminobutyric acid (GABA) receptors)

Ligandin (Y protein) The Bilirubin-binding protein at the surface of the liver. Bilirubin bound to ligandin is actively transported to the smooth endoplasmic reticulum where it is rendered water soluble by conjugation with glucuronate

Ligase Enzymes catalysing condensation reactions using the energy of ATP or some other high energy compound see DNA ligase

Ligase chain reactions A technique for detecting DNA sequence variations between alleles. Two primers anneal at adjacent sites on target DNA. If the target sequence is present, the primers can be ligated together and function as sites for subsequent annealing and ligation. This results in amplification of the primer ligation product. If the target sequence is absent, ligation and amplification do not occur. Conditions can be chosen such that only exactly complementary primers anneal. This permits discrimination between alleles that differ by a single point mutation (allele-specific ligation)

Ligate The process of joining two or more DNA fragments

Ligation Joining of two DNA molecules by the formation of phosphodiester bonds. This reaction is catalysed by DNA ligase. In gene cloning, a foreign DNA (DNA insert) is ligated to a linearized vector molecule using T4 DNA ligase

Light chain One of the smaller polypeptides of a multisubunit protein such as immunoglobulin or myosin. In immunoglobulins, it is abbreviated at L chain

Light reactions The reactions of photosynthesis that requires light

Light-harvesting complex (LHC) A complex of pigments associated with the photochemical reaction centres in chloroplasts. The pigments include carotenoids, phycobilins, phycoerythrins and chlorophylls. The LHC collects photons that fall on an area of the thylakoid disc with transfer of the photon energy to chlorophyll in the form of an excited electron and the energy is stored as ATP or NADPH. LHCs contain several pigments that function as antennae for collecting light

Lignification Impregnation of a cell wall with lignin.

Lignin A group of high-molecular-weight polymers of phenlypropanoid compounds, that congers strength to certain tissues such as wood. Wood contains lignified xylem cells.

Lignocellulose The combination of lignin, hemicellulose and cellulose which together makes up the structural framework of plant cell walls.

Limit dextrins The products formed by the action of amylase on amylopectin.

Limit digest The product of a degradative enzyme reaction in which the substrate is completely digested. The extent of digestion may be limited by physical constraints such as clumping of the substrate or failure of the substrate to enter into solution. Such as clumping of the substrate or failure of the substrate to enter into solution.

Limit(ing) dilution A technique used to count the number of functional cells in a population.

LIMS (Laboratory Information Management Systems) System that manage output form high-throughput sequencing.

Lincomycin (lincocin) An antibiotic produced by Streptomyces lincolnesis that binds to the large subunit of the ribosome and prevents elongation of the polypeptide chain during protein biosynthesis.

Lineage Several individuals originating from a common descent. For example, the generation of a cell line from a single cell plated in vitro.

Linear phase The phase between the exponential growth and the deceleration phases when cell numbers constantly increase. See growth phases.

LINEs Long interspersed nuclear elements. These are families of long (average length = 6500 bp), moderately repetitive (about 10,000 copies) sequences present in mammalian genomes. LINEs are CDNA copies of functional genes found in the same genome.

Lineweaver-Burk Equation A mathematical transformation of the Michaelis-Menten equation that allow the determination of maximum velocity (Vmax) and Km by extrapolating substrate concentration [S] to infinity.

Lineweaver-Burk plot A plot of 1/vi against 1/[S], to give a straight line with a slope of Km/Vmax. Also known as double reciprocal plot.

The Lineweaver-Burk Plot

The Lineweaver-Burk Plot



Linkage
The tendency of two or more genes to be inherited together because of their location on the same chromosome. Linkage is greater when two genes are located sufficiently close on the same chromosome.

Linkage analysis Mapping the relative position of two genes or markers based on estimating the degree to which they are inherited together.

Linkage disequilibrium A condition in which certain combinations of genetic markers occur more frequently in the population than expected when considering their relative distance. This signifies coordinate inheritance of a group of markers. This may be due to reduced recombination in the region or founder effect, because of which there has been insufficient time to reach equilibrium since one of the markers was introduced into the population.

Linkage group all the loci that can be connected by linkage relationship. This is equivalent to a chromosome.

Linkage map A linear or circular diagram that indicates the relative positions of genes on a chromosome as determined by recombination fraction. See genetic map.

Linkage genes; linked markers Genes or markers that show linkage.

Linker A short, synthetic double-stranded oligonucleotide that contains the recognition sequence for one or more restriction endonuclease sites. It is added to blunt-ended DNA to facilitate splicing.

Linker DNA The DNA that connects two nucleosome core particles. It excludes the 146 bp of DNA that is wrapped around the core histones.

Linker histone Histone H1. A large molecule of mw 23 kDa that stabilises DNA between the nucleosome cores and plays a key role in converting chromatin to the highly condensed chromosome during cell division. A nucleosome core together with H1 is called a chromosome during cell division. A nucleosome core together with H1 is called a chromatosome. In some cell types. H1 is replaced by histone H5, which binds tightly to chromatin. It is associated with DNA that is not being transcribed.

Linker scanner mutations Mutations that are introduced by recombining two DNA molecules in vitro at a restriction fragment added to the end of each fragment. This inserts the linker sequence at the site of recombination.

Left Right