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Home >> Dictionary of Bioinformatics, Biochemistry, Biotechnology >> Guanosine - Haemagglutination Inhibition Assay

GTP-binding proteins
See G proteins.

Guanethidine
A drug that decreases catecholamine levels by increasing their catabolism. This is useful as an antihypertensive agent.

Guanidine
H2N-C(=NH2)-NH2. A denaturing agent at high concentrations.

Gynandromorphy
An individual in which one part of the body is female and the other is male; a sex mosaic.

Guanine
A purine base found in nucleotides, DNA and RNA.

Guanine

Guanine


Guanine nucleotide exchange factor (GEF)
A protein that binds to a G-protein and activates it by stimulating it to release the tightly bound GDP and permitting it to bind GTP in its place.

Guanosine triphosphate (GTP)
Guanosine containing three phosphate groups linked to the fifth carbon atom of the ribose moiety. GTP together with other nucleotides, is a precursor of RNA. It is a high-energy compound that provides energy for biosynthetic reactions.

Guessmer
A short synthetic oligonucleotide deduced to be the most likely DNA sequence from the amino acid sequence data.

GUI
Graphical user interphase. A higher-level operating system that has a more sophisticated user interface than the basic text-input style of a lower-level operating system. The Windows desktop is a classic examples of a GUI.

Guide sequence
An RNA molecule that hybridises with eukaryotic mRNA and helps in the removal of introns. Guide sequences may be either external (EGS) or internal (IGS) to the RNA, which is being processed and may hybridise with either intron or exon sequences close to the splice junction. See split gene; exon.

Guilt by association-expression
A systematic approach for assessing gene function based on the observation that genes that are related tend to cluster together based on their experimentally determined expression patterns.

Guide RNA
An RNA molecule that contains sequences that function as a template during RNA editing.

Gunzberg’s test
A test to confirm the presence of free acid in gastric juice. A few drops of gastric contents mixed with equal amounts of Gunzberg’s reagent (phloroglucinol and vanillin in ethanol) and evaporated to dryness on a water bath, leaves a reddish colour if free hydrochloric acid is present.

Gynaecomastia
Excessive development of the mammary glands in males.

Guanosine
A nucleoside made up of a guanine molecule linked to a ribose. The nucleotides derived from guanosine molecule linked to a ribose. The nucleotides derived from Guanosine mono-, di-, and triphosphate (GMP, GDP and GTP respectively), play key roles in various metabolic reactions.

Guanosine

Guanosine


Guanosine monophosphate (GMP)
A derivative of guanosine formed by Phosphorylation of the 5th carbon of ribose.

Gynogenotes
Zygotes containing two female nuclei.

Gyrase
A type II topoisomerase of E.coli that introduces negative supercoils into DNA.

gz
A file extension for UNIX files that is compressed by using the utility gzip. See zip.

H+
See proton.

H-DNA
A triple helical structure formed by base pairing and interwinding of three strands of DNA. These sequences are present in regions involved in regulation of expression of many genes in eukaryotes. Formation of H-DNA may play a role in eukaryotic gene expression.

H-DNA

H-DNA


H5N1
See avian flu.

H-2 complex
The major histocompatibility complex situtated on chromosome 17 of the mouse. It contains subregions K, I and D

H-2histocompatbility
The match of tissue proteins that determines whether a graft will be accepted or rejected by the host. H-2 histocompatibility is determined by a large gene complex that encodes cell surface glycoproteins.

HA protein
Haemagglutinin protein. A glycoprotein present on the surface of the influenza virus that binds to sialic acid residues on the cell membranes of viral infected cells and mediates fusion between the viral membrane and a membrane of an endosome that encapsulates the virus.

Haber-Weiss reaction
The action in which hydroxyl ion is formed by the breakdown of H2O2 in the presence of transition metal ions such as Fe2+. Also known as Fenton reaction.

Habituation
The property of some cells to grow by themselves without the addition of specific factors after a number of subcultures. Such cells are autonomous and no longer need exogenous growth regulators in the tissue culture medium.

2-HBD (2-hydroxybutyrate dehydrogenase)
A isoenzyme of lactate dehydrogenase (LD). LD catalyses the oxidation of 2-hydroxybutyrate to 2-oxobutyrate. HBD activity is a sensitive index of myocardial infarction with decrease in LD/HBD ratio to between 0.8-1.2 (1.2-1.6 in healthy individuals).

Haem
Iron-porphyrin. The haem moiety in haemoglobin is protoporphyrin IXa, a tetrapyrrole ring system linked to ferrous ion.

The Structure of Haem
M = methyl, V = vinyl, P = propionate
The Structure of Haem


Haem protein
A protein that contains haem as a prosthetic group.

Haemagglutination inhibition assay
An assay that detects the presence of a haemagglutinating virus or other antigen based on the loss of the ability of a test sample to agglutinate red blood cells after treatment with an antibody against the suspected agglutinin.

Haemagglutinin/Neuraminidase protein (HN Protein)
A substance that binds to certain sites on red blood cell (RBC) membrane and causes agglutination. Haemagglutination is widely used as an assay for the presence of certain haemagglutinating viruses or other antigens.

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