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Home >> Dictionary of Bioinformatics, Biochemistry, Biotechnology >> Fluidised Bed, FMN (Flavin Mononucleotide)

Fluctuation analysis A method developed by Salvador Luria and Max Delbruck in 1943 to demonstrate that mutations occur spontaneously in bacteria.

Fluid mosaic model A model of the eukaryotic cell membrane proposed by Singer and Nicolson in 1972 based on the concept that the membrane is a semisolid lipid bilayer into which integral and peripheral proteins are embedded.

Fluor Materials that convert kinetic energy into flashes of light or scintillations when exposed to ionizing radiation.

Fluorescamine A fluorescent stain used for detecting amino acids, peptides and proteins.

Fluorescein dilaurate test A pancreatic function test. When fluorescein dilaurate is administered orally, it is hydrolysed in the gut by pancreatic esterase. The fluorescein released is absorbed from the gut, conjugated in the liver and excreted as glucuronide in the urine. Fluorescein dilaurate (0.5 mmol) is given orally with adequate fluid intake. The amount of fluorescein excreted is measured in the urine sample collected for 10 h. An equivalent amount of free fluorescein is administered the following day as control. If pancreatic function is normal, the test/control index is greater than 30 per cent. In pancreatic insufficiency, the index is less than 20 per cent.

Fluorescence The phenomenon by which a molecule absorbs radiation and emits it at longer wavelength.

Flourescence-activated cell sorting (FACS) The use of laser beams to detect differences in fluorescence between different types of cells in a mixture. This is followed by deflection of each cell type into separate bins. This technique is used for sperm sexing.

Fluorescence microscope A microscope designed to view samples stained with fluorescent dyes. The illuminating light is passed through a set of filters before the specimen to select a particular wavelength that excites the dye and through another set of filters before it reaches the eye piece in order to select only the wavelength emitted when the dye fluoresces.

Fluorescence scanners A fluorescence-based detection method used with microarrays, high-density oligonucleotide arrays, and microelectronic chips. Fluorescence is generally detected with a confocal scanning microscope.

Fluorescent antibody An antibody coupled to a fluorescent dye, used with a fluorescence microscope to detect antigen on cells, tissues, or microorganisms.

Fluorescent antibody techniques The techniques for the localization of a particular antigen in a tissue or cell preparation based on the biding of an antibody with a fluorescent label to the antigen of interest.

Fluorescent dye A molecule that absorbs light at one wavelength and emits it at another longer wavelength of lower energy. Fluorescein, which emits green light, is a commonly used fluorescent dye. Also called fluorochrome.

Fluorescent green protein See fgr.

Fluorescent label A molecule that fluoresces and can be attached to another nonfluorescing probe molecule such as an antibody or a DNA probe.

Fluorescent probe A probe whose response is based on the intensity of fluorescence emitted by individual cells or cell components.

Fluorescence immunoassay An immunoassay technique which combines the specificity of antibodies wit the sensitivity of fluorimetric assays by using antibodies coupled to a fluorescent chromophore.

Fluorescence in situ hybridization See FISH.

Fluorescent resonance energy transfer (FRET) A technique for evaluating the closeness of two fluorescently labelled molecules and their interactions in the cells.

Fluorescent staining A technique for the visualization of cellular components by treating cells or tissues with a fluorochrome-labelled agent such as an antibody, which binds to a specific tissue component followed by observation under a fluorescence microscope

Fluidity A property of membranes. It indicates the ability of membrane lipids to move laterally within the particular monolayer.

Fluidized bed Use of particles to support enzyme molecules or cells in bioreactors. There are several methods to ensure smooth and uniform flow of reactants past such a bed. In fluidized through it. It is used in the industrial processing of gases. In entrained bed reactors, the approach are used in cell culture systems. In packed bed reactors, the particles are packed into a bed and remain solid. This is used in chromatography columns

Fluorimetry The quantitative measurement if fluorescence.

Fluorochrome See fluorescent dye.

5-Fluorodeoxyuridine The nucleotide derivative of 5-fluorouracil formed in cells treated with 5-fluorouracil. Both 5-fluorouracil and 5-fluorodeoxyuriding are used as anticancer agents.

5-Fluorouracil (5-FU) An analogue of uracil that can be converted to 5-fluorodeoxyuridine 5’-monophosphate (FdUMP), which is the form exhibiting antineplastic activity. FdUMP interacts with thymidylate synthase as well as N5,N10-methylene tetrahydrofolate and blocks synthesis of dTMP and DNA synthesis.

Fluorouracil


Flush end See blunt end.

Flush-end cut See blunt-end cut.

Fluxome A recent methodology for metabolic flux ratio (METAFoR) analysis. This can directly reveal active metabolic pathways. Generation of fluxome data arrays by use of the METAFoR approach is based on two-dimensional 13C-1H correlation nuclear magnetic resonance spectroscopy with fractionally labelled biomass.

FMN (flavin monoucleotide) Riboflavin phosphate, a coenzyme for some redox reactions.

Flavin Mononucleotide


Fms The oncogene carried by the McDonnough strain of feline sarcoma virus.

Focal adhesion (adhesion plaque) A type of anchoring cell junction that forms a small region on the surface of a fibroblast or other cell anchored to the extracellular matrix. The attachment is mediated by integrins.

Focal adhesion kinase (FAK) A cytoplasmic tyrosine kinase found at cell-matrix junctions (focal adhesions) to gether with the cytoplasmic tails of integrins.

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