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Home >> Dictionary of Bioinformatics, Biochemistry, Biotechnology >> Enzyme Commission (EC) Number, Epistatic Gene

Enzyme cascade A series of reactions in which one enzyme activates the other resulting in amplification of the signal that initiated the cascade. Such activation usually occurs by phosphorylation and involves regulatory events.

Enzyme Commission (EC) Number Systematic name and number for the identification of an enzyme. The EC number is assigned by the Enzyme Commission. It consists of four numbers separated by dots: the first number classifies the enzyme into one of the six major groups: 1. Oxidoreductases; 2. Transferases; 3. Hydrolases;

4. Lyases; 5. Isomerases; 6. Ligases. Each group is subdivided into subgroups, each subgroup into subsub-groups, and the last number is specific for the enzyme. For example, EC 3.1.21.1 is deoxyribonuclease I.

Enzyme derepression The induction of enzyme activity by removing or inactivating an inhibitor. For example, induction of b-galactosidase

Enzyme electrode A type of biosensor, in which the biological component is an enzyme which is immobilised onto the surface of an electrode. When the enzyme catalyses its reaction, electrons are transferred from the reactant to the electrode generating current. See biosensor.

Enzyme kinetics Investigation of the binding rate of an enzyme to its substrate.

Enzyme-linked immunosorbent assay (ELISA) A technique for detecting a specific molecule in a sample. A primary antibody binds to a target molecule. A secondary antibody that can bind to the primary antibody is subsequently added. The secondary antibody is attached to an enzyme that can convert a colourless substrate to a coloured product. If the target molecule is present in the sample, a coloured product is formed that can be detected. ELISA is used for diagnostic and research purposes.

Enzyme-linked receptor A type of cell-surface receptor in which the cytoplasmic domain itself has enzymatic activity or is associated with an intracellular enzyme. Enzymatic activity in both the cases is induced by the ligand binding to the receptor.

Enzyme replacement therapy A therapeutic method by which a defective enzyme is replaced with a functional enzyme.

Enzyme stabilization The inhibition of enzyme inactivation by altering the salt concentration, pH or lowering the temperature of an enzyme solution. Modification of enzymes by attachment of organic groups or altering the amino acid composition of the enzyme are used to stabilize enzymes.

Eosinophil chemotactic factor of anaphylaxis (ECF-A) A substrate released from mast cells during anaphylaxis that attracts eosinophils.

Epidemiology The analysis of the occurrence of a disease in a population with respect to distribution, incidence and causative factors.

Epidermal growth factors (EFGs) A family of secreted signalling proteins that play a role in tissue development in most animals. The receptors for these growth factors are tyrosine kinases. Mutations in EGF signal transduction have been reported in human cancer.

Enzymome Assay of all proteins predicted from a  proteome for potential enzymatic activities.

Eosinophil A subclass of leucocytes that stain intensely with eosin. These are amoeboid cells similar to macrophages and found in large number in blood of individuals with parasitic infection.

Epidermis 1. The outmost layer of cells of the body of an animal. 2. The outermost layer of cells covering a plant. It is covered by a cuticle. Some epidermal cells are modified to form guard cells or hair of various types. In both plants and animals, the principal function of the epidermis is protection against injury and to reduce water loss

Enzyme engineering Modification of enzymes by recombinant DNA technology and site-directed mutagenesis. The modifications include increasing protein stability and catalytic activity and/or substrate specificity, changing the optimal requirements for catalysis to enable the engineered enzymes to function under nonphysiological conditions and/or become resistant to feedback regulation. These enzymes are used for industrial purposes.

Enzyme immobilization The chemical bonding of an enzyme to a solid matrix in such a way that the enzyme activity is preserved. This is an essential step in the development of enzyme-based biochemical assays.

Epididymis A system of tubules that emerges from the tests and functions as a storage site for sperms during their maturation.

Epigenesis A theory that considers that development is an literative series of steps in which various signals and control events interact to regulate the process.

Epigenetic Changes that influence the phenotype without altering the genotype.

Epigenetic imprinting Refers to drug – or environment-induced changes in gene expression that is indicative of disease and/or pharmacological or environmental exposure.

Epigenomics The study of complex expression networks or linkages both spatially (within the body) and temporally (at different times in development).

Epigenotype A methylation state of DNA.

Epimerase An enzyme that catalyses the conversion of one epimer to another.

Epimers Two stereoisomers containing more than one chiral centre differing in configuration at one of their chiral centres.

Epimutation A change in the methylation state of DNA.

Epinephrine (Adrenaline). A hormone synthesised and secreted by the adrenal cortex in response to stress. It increases glycogenolysis, gluconeogenesis, glycolysis and lipolysis.

Epinephrine



Epipodophyllotoxins etoposide (VP-16) An anticancer agent derived from roots of May apple that acts by interrupting DNA topoisomerase II.

Episialin A high molecular weight glycoprotein expressed by mammary epithelium that can be detected by the carbohydrate markers CA 15-3, CA459 and CA27.29 which serve as markers for carcinoma of the breast.

Episome A plasmid capable of integrating into the chromosome. For exmple, the fertility factor (F) in Escherichia coli.

Epistasis A condition in which the expression of one gene masks the phenotypic effects of another gene. The suppressed genes are said to be hypostatic.

Epistatic gene A gene that suppresses the effect of another, nonallelic gene. See allele.

Epithelium A tissue that forms the outer covering of an organ or organism.

Epitomics The study of all epitopes of the proteome in an organism. See epitope.

Epitope A specific chemical domain on an antigen that is recognised by an antibody. Each epitope on a molecule is capable of inducing synthesis of a different antibody. Also called antigenic determinant.

Epizootic A disease that affects a large number of animals simultaneously.

Epstein-Barr virus (EBV) A member of the herpes family of DNA viruses implicated in causing Burkitt’s lymphoma in West Africa and New Guinea. It infects B cellsb

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