The effectiveness of vaccines may be appreciated from the fact that smallpox, once a dreaded disease the world over, has been completely, eradicated from the world; the last case of smallpox was reported in 1977.
An accurate diagnosis of the disease and its causal organism is critical to its effective management and cure. Conventionally disease diagnosis is based on the following:
i. Microscopic examination
ii. Culture of specimen
iii. Immunological assays
iv. Detection and measurement of the pathogen-specific antibodies
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